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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was identified to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two added splice variants had been claimed but detailed nucleotide sequence data is just not nonetheless readily available.
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Blocking peptides are peptides that bind exclusively to the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This tutorial outlines even further achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the very best benefits are...
Antibodies certain to the blocking peptide no longer bind to the epitope around the target protein. By comparing the staining in the blocked antibody as opposed to the antibody by yourself, one can see which staining is specific.
Concentrate on Facts Cyclin E belongs to your really conserved cyclin household, whose customers are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show distinctive expression and degradation styles which contribute to your temporal coordination of every mitotic occasion. Cyclin E kinds a fancy with and functions being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates in the G1-S period boundary and it is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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